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human pd  (ACROBiosystems)


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    ACROBiosystems human pd
    Human Pd, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pd/Human+PD-1+%2F+PDCD1+Protein%2C+His+Tag/pm42014806-86-9-13
    Average 95 stars, based on 94 article reviews
    human pd - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Binding Assay:

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer by antagonizing the PD-L1/PD-1 interaction
    Article Snippet: .. The published K D values for human PD-1/PD-L1 binding measured using titration ELISA ranges from 10 to 50 nM (technical notes from Cisbio and ACROBiosystems), whereas the apparent K D of VSTM2A binding with PD-L1 ranges from 0.7 to 2.5 nM, suggesting that the binding affinity of VSTM2A/PD-L1 is higher. ..

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer through antagonizing PD-L1/PD-1 interaction.
    Article Snippet: .. The 251 published Kd values for human PD-1/PD-L1 binding measured using titration ELISA ranges 252 10- 50 nM (Technical notes from Cisbio and ACROBiosystems), whereas the apparent Kd of 253 VSTM2A binding with PD-L1 ranges 0.7-2.5 nM, suggesting the binding affinity of 254 VSTM2A/PD-L1 is higher. ..

    Titration:

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer by antagonizing the PD-L1/PD-1 interaction
    Article Snippet: .. The published K D values for human PD-1/PD-L1 binding measured using titration ELISA ranges from 10 to 50 nM (technical notes from Cisbio and ACROBiosystems), whereas the apparent K D of VSTM2A binding with PD-L1 ranges from 0.7 to 2.5 nM, suggesting that the binding affinity of VSTM2A/PD-L1 is higher. ..

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer through antagonizing PD-L1/PD-1 interaction.
    Article Snippet: .. The 251 published Kd values for human PD-1/PD-L1 binding measured using titration ELISA ranges 252 10- 50 nM (Technical notes from Cisbio and ACROBiosystems), whereas the apparent Kd of 253 VSTM2A binding with PD-L1 ranges 0.7-2.5 nM, suggesting the binding affinity of 254 VSTM2A/PD-L1 is higher. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer by antagonizing the PD-L1/PD-1 interaction
    Article Snippet: .. The published K D values for human PD-1/PD-L1 binding measured using titration ELISA ranges from 10 to 50 nM (technical notes from Cisbio and ACROBiosystems), whereas the apparent K D of VSTM2A binding with PD-L1 ranges from 0.7 to 2.5 nM, suggesting that the binding affinity of VSTM2A/PD-L1 is higher. ..

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Article Title: MUC1-targeted CAR-T cell secreted anti-PD-1 IgG antibody enhances antitumor activity in Cholangiocarcinoma.
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) AR TIC LE IN PR ES S To evaluate the binding of secreted anti-PD-1 Ig, culture supernatant from CAR-T cells was collected on day 4 following transduction and stored at -20°C. .. In brief, 96 well ELISA plates are coated with Human PD-1/PDCD1 Protein, Histag (ACROBiosystems, cat# PD1-H522a) at 50 ng per well in 100 μl PBS at 4°C overnight. ..

    Article Title: VSTM2A reverses immunosuppression in colorectal cancer through antagonizing PD-L1/PD-1 interaction.
    Article Snippet: .. The 251 published Kd values for human PD-1/PD-L1 binding measured using titration ELISA ranges 252 10- 50 nM (Technical notes from Cisbio and ACROBiosystems), whereas the apparent Kd of 253 VSTM2A binding with PD-L1 ranges 0.7-2.5 nM, suggesting the binding affinity of 254 VSTM2A/PD-L1 is higher. ..

    Activity Assay:

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Recombinant:

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Article Title: Bispecific killer engager for targeted depletion of PD-1 positive lymphocytes: A new avenue for autoimmune disease treatment
    Article Snippet: Annexin V (Ref. A35110); PI (Ref. P3566); anti-mouse IgG2a-HRP conjugate (Y2a) were purchased from Invitrogen (Eugene, OR, USA); APC-anti-human IgG Fc (G18145) was purchased from BD Biosciences (San Jose, CA, USA). .. Human CD16a recombinant protein-His (cat. F176) and human PD-1 recombinant protein-mouse Fc Tag (cat. PD-1-H5255) were purchased from AcroBiosystems (Newark, DE, USA); CCK8 metabolic assay (ab228554) was purchased from Abcam (Eugene, OR, USA). .. SuperSignal ELISA Pico Chemiluminescent Substrate (Ref. 37069) was purchased from ThermoFisher Scientific (Euguene, OR, USA).

    Multi-Angle Light Scattering:

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Concentration Assay:

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. To detect the secretion and binding activity of anti-PD1 scFv, ELISA plates were coated overnight at 4 °C with recombinant human PD-1/PDCD1 protein, Fc tag, low endotoxin (MALS verified) (ACROBiosystems, Catalogue No. H5257-100 μg) at a concentration of 50 ng per well in 100 μL of 1xPBS (tested range: 10–100 ng/well). ..

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. For full-Anti-PD1 detection, human PD-1/PDCD1 Protein, His-tag (ACRO Biosystems, cat# PD1-H522a) was incubated overnight at 4 °C at a total concentration of 50 ng per well in 100 μL of 1xPBS. .. The next day, plates were washed three times with PBS containing 0.05% Tween-20 (PBST) and incubated with 200 μL of culture supernatants, followed by two-fold serial dilutions at 37 °C for 1 h. After washing, wells were incubated with HRP-conjugated goat anti-human IgG Fc antibody (Invitrogen, cat# A55745 ; 1:8000 dilution in PBST) for 1 h at 37 °C.

    Metabolic Assay:

    Article Title: Bispecific killer engager for targeted depletion of PD-1 positive lymphocytes: A new avenue for autoimmune disease treatment
    Article Snippet: Annexin V (Ref. A35110); PI (Ref. P3566); anti-mouse IgG2a-HRP conjugate (Y2a) were purchased from Invitrogen (Eugene, OR, USA); APC-anti-human IgG Fc (G18145) was purchased from BD Biosciences (San Jose, CA, USA). .. Human CD16a recombinant protein-His (cat. F176) and human PD-1 recombinant protein-mouse Fc Tag (cat. PD-1-H5255) were purchased from AcroBiosystems (Newark, DE, USA); CCK8 metabolic assay (ab228554) was purchased from Abcam (Eugene, OR, USA). .. SuperSignal ELISA Pico Chemiluminescent Substrate (Ref. 37069) was purchased from ThermoFisher Scientific (Euguene, OR, USA).

    Incubation:

    Article Title: A Bidirectional EF1 Promoter System for Armoring CD19 CAR-T Cells with Secreted Anti-PD1 Antibodies
    Article Snippet: .. For full-Anti-PD1 detection, human PD-1/PDCD1 Protein, His-tag (ACRO Biosystems, cat# PD1-H522a) was incubated overnight at 4 °C at a total concentration of 50 ng per well in 100 μL of 1xPBS. .. The next day, plates were washed three times with PBS containing 0.05% Tween-20 (PBST) and incubated with 200 μL of culture supernatants, followed by two-fold serial dilutions at 37 °C for 1 h. After washing, wells were incubated with HRP-conjugated goat anti-human IgG Fc antibody (Invitrogen, cat# A55745 ; 1:8000 dilution in PBST) for 1 h at 37 °C.



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    Image Search Results


    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model ADCC, PD‐L1 was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.

    Journal: Clinical & Translational Immunology

    Article Title: Parallel processing of T cells and natural killer cells to enhance in vivo CAR T cell activity against blood and solid cancers

    doi: 10.1002/cti2.70120

    Figure Lengend Snippet: Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model ADCC, PD‐L1 was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.

    Article Snippet: The human PD‐L1 (NCBI 29126) gene with endogenous signal sequence was synthesised by Twist Biosciences and cloned into SfiI sites of pSBbiP—a modified pSBbiGP encoding puromycin resistance but with the original GFP gene removed.

    Techniques: Generated, Marker, Cytotoxicity Assay

    Recycled natural killer (NK) cells enhance CAR T cell therapy of MCF‐7. NSG mice were engrafted with Her2 h i PDL1 hi MCF‐7 cells on Day 0 prior to sequential administration of NK cells (d10) and CAR T cells (d12) (a) . Quality control of both cell types ensured high NK cell purity and transduction efficiency of T cells prior to administration (b, c) . Mice were imaged weekly (d, e) , and callipers were used every other day to monitor tumor burden (f) . Mice were euthanised when radiance and/or tumor volume reached endpoint thresholds (g) . Data from one mouse experiment. n = 4–5 mice per group. Tumor burdens displayed as mean ± SEM. * P < 0.05 by two‐way ANOVA with Bonferroni correction. Survival analysed with Kaplan–Meier, log‐rank mantel‐cox test. ** P ≤ 0.005. White out‐lined box conceals mouse removed from study because of failed tumor engraftment.

    Journal: Clinical & Translational Immunology

    Article Title: Parallel processing of T cells and natural killer cells to enhance in vivo CAR T cell activity against blood and solid cancers

    doi: 10.1002/cti2.70120

    Figure Lengend Snippet: Recycled natural killer (NK) cells enhance CAR T cell therapy of MCF‐7. NSG mice were engrafted with Her2 h i PDL1 hi MCF‐7 cells on Day 0 prior to sequential administration of NK cells (d10) and CAR T cells (d12) (a) . Quality control of both cell types ensured high NK cell purity and transduction efficiency of T cells prior to administration (b, c) . Mice were imaged weekly (d, e) , and callipers were used every other day to monitor tumor burden (f) . Mice were euthanised when radiance and/or tumor volume reached endpoint thresholds (g) . Data from one mouse experiment. n = 4–5 mice per group. Tumor burdens displayed as mean ± SEM. * P < 0.05 by two‐way ANOVA with Bonferroni correction. Survival analysed with Kaplan–Meier, log‐rank mantel‐cox test. ** P ≤ 0.005. White out‐lined box conceals mouse removed from study because of failed tumor engraftment.

    Article Snippet: The human PD‐L1 (NCBI 29126) gene with endogenous signal sequence was synthesised by Twist Biosciences and cloned into SfiI sites of pSBbiP—a modified pSBbiGP encoding puromycin resistance but with the original GFP gene removed.

    Techniques: Control, Transduction

    Elevated ABHD16A triggered by ACh was associated with poor prognosis in patients with gastric cancer. A, Representative IHC images of ABHD16A expression in human normal gastric tissues and gastric cancer tissues from the TMA with magnifications of 40×, 100×, and 200×. Scale bar for 200×, 100 μm. B–D, Relationship of ABHD16A IHC score to clinical stage ( B ), pT stage ( C ), and lymph node metastasis ( n = 80; D ). E, Cumulative survival curves of patients with gastric cancer with high or low ABHD16A expression based on the TMA. F, IHC images and score of ABHD16A in S100 + or S100 − gastric cancer tissues. Scale bar, 100 μm. G, Procedure for coculture of DRG neurons and gastric cancer cells. H, Western blotting was used to analyze ABHD16A levels in MFC cells cocultured with DRG neurons or DRG neuron–derived CM. GAPDH served as the loading control. I, Expression of HIF1A and ABHD16A in neurotransmitter [HA, dopamine (DA), 5-HT, norepinephrine (NE), ACh]-treated MFC cells and ACh-treated MGC-803 cells. J, ACh concentration was detected in DRG CM by ELISA. K, Tumor volume of orthotopic gastric cancer tumors with or without vagotomy. L, Representative mIF staining images of FOXP3, CD163, CD11b, CD8, PD-L1, and Pan-CK in gastric cancer tissues with high or low expression of ABHD16A. Scale bar, 100 μm. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

    Journal: Cancer Research

    Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

    doi: 10.1158/0008-5472.CAN-25-3092

    Figure Lengend Snippet: Elevated ABHD16A triggered by ACh was associated with poor prognosis in patients with gastric cancer. A, Representative IHC images of ABHD16A expression in human normal gastric tissues and gastric cancer tissues from the TMA with magnifications of 40×, 100×, and 200×. Scale bar for 200×, 100 μm. B–D, Relationship of ABHD16A IHC score to clinical stage ( B ), pT stage ( C ), and lymph node metastasis ( n = 80; D ). E, Cumulative survival curves of patients with gastric cancer with high or low ABHD16A expression based on the TMA. F, IHC images and score of ABHD16A in S100 + or S100 − gastric cancer tissues. Scale bar, 100 μm. G, Procedure for coculture of DRG neurons and gastric cancer cells. H, Western blotting was used to analyze ABHD16A levels in MFC cells cocultured with DRG neurons or DRG neuron–derived CM. GAPDH served as the loading control. I, Expression of HIF1A and ABHD16A in neurotransmitter [HA, dopamine (DA), 5-HT, norepinephrine (NE), ACh]-treated MFC cells and ACh-treated MGC-803 cells. J, ACh concentration was detected in DRG CM by ELISA. K, Tumor volume of orthotopic gastric cancer tumors with or without vagotomy. L, Representative mIF staining images of FOXP3, CD163, CD11b, CD8, PD-L1, and Pan-CK in gastric cancer tissues with high or low expression of ABHD16A. Scale bar, 100 μm. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

    Article Snippet: FITC anti-human CD3 (Thermo Fisher Scientific, cat. #11-0038-42, RRID: AB_2043831, 5 μL/1 × 10 6 cells), PE anti-human CD127 (BioLegend, cat. #351304, RRID: AB_10720185, 5 μL/1 × 10 6 cells), Brilliant Violet 421 anti-human CD294 (BioLegend, cat. #350112, RRID: AB_2562468, 5 μL/1 × 10 6 cells), APC anti-human CD117 (BioLegend, cat. #313206, RRID: AB_314985, 5 μL/1 × 10 6 cells), FITC anti-human CD19 (eBioscience, cat. #11-0199-42, RRID: AB_10669461, 5 μL/1 × 10 6 cells), FITC anti-human CD14 (eBioscience, cat. #11-0149-42, RRID: AB_10597597, 5 μL/1 × 10 6 cells), BV650 anti-human CD45 (eBioscience, cat. #416-0459-42, RRID: AB_2925684, 5 μL/1 × 10 6 cells), iFluor 647 anti-Ki67 (HUABIO, cat. #HA720163F, RRID: AB_3072100, 1 μL/1 × 10 6 cells), PE/Cyanine7 anti-human CD274 (Elabscience, cat. #E-AB-F1133H, 5 μL/1 × 10 6 cells), and PE anti-human CD273 (Elabscience, cat. #E-AB-F1175D, 5 μL/1 × 10 6 cells).

    Techniques: Expressing, Western Blot, Derivative Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining

    ABHD16A promotes ILC3 aggregation and IL22 release in the immune microenvironment. A, A total of 5 × 10 6 MFC-luc or Abhd16a -knockdown MFC-luc cells were injected into the epidermis of the greater curvature of the stomach in the 615 mice. Tumor growth was monitored weekly through bioluminescence imaging from the first day of MFC cell inoculation. Scale bar, 2.000e+4 – 5.000e + 5 p/s/cm 2 /sr. B and C, Representative images of the excised stomachs ( B ) and the orthotopic tumor volume ( C ) on day 28 after gastric cancer cells were implanted orthotopically ( n = 9 per group). D, H&E images of the stomach, peritoneum, liver, intestine, and lung on day 42 after MFC or Abhd16a -knockdown MFC cells were implanted into 615 mice. Scale bar, 400 μm. E–G, Representative images ( E ), volume ( F ), and weight ( G ) of control and Abhd16a -knockdown subcutaneous tumors ( n = 5 per group). H, IHC images of PD-L1 in control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tissues. Scale bar, 500 μm. I, Detection of mouse cytokines in the TIF of orthotopic gastric cancer tissues by proteome profiler mouse XL cytokine array. J, Flow cytometry gating strategy and frequencies of ILC3s and Th17/Th22 cells out of CD45 + cells in control and Abhd16a -knockdown gastric cancer tissues in 615 mice. K, Flow cytometry gating strategy and frequencies of ILC3s out of ILCs in control and Abhd16a -knockdown gastric cancer tissues in Rag1 −/− mice. ***, P < 0.001.

    Journal: Cancer Research

    Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

    doi: 10.1158/0008-5472.CAN-25-3092

    Figure Lengend Snippet: ABHD16A promotes ILC3 aggregation and IL22 release in the immune microenvironment. A, A total of 5 × 10 6 MFC-luc or Abhd16a -knockdown MFC-luc cells were injected into the epidermis of the greater curvature of the stomach in the 615 mice. Tumor growth was monitored weekly through bioluminescence imaging from the first day of MFC cell inoculation. Scale bar, 2.000e+4 – 5.000e + 5 p/s/cm 2 /sr. B and C, Representative images of the excised stomachs ( B ) and the orthotopic tumor volume ( C ) on day 28 after gastric cancer cells were implanted orthotopically ( n = 9 per group). D, H&E images of the stomach, peritoneum, liver, intestine, and lung on day 42 after MFC or Abhd16a -knockdown MFC cells were implanted into 615 mice. Scale bar, 400 μm. E–G, Representative images ( E ), volume ( F ), and weight ( G ) of control and Abhd16a -knockdown subcutaneous tumors ( n = 5 per group). H, IHC images of PD-L1 in control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tissues. Scale bar, 500 μm. I, Detection of mouse cytokines in the TIF of orthotopic gastric cancer tissues by proteome profiler mouse XL cytokine array. J, Flow cytometry gating strategy and frequencies of ILC3s and Th17/Th22 cells out of CD45 + cells in control and Abhd16a -knockdown gastric cancer tissues in 615 mice. K, Flow cytometry gating strategy and frequencies of ILC3s out of ILCs in control and Abhd16a -knockdown gastric cancer tissues in Rag1 −/− mice. ***, P < 0.001.

    Article Snippet: FITC anti-human CD3 (Thermo Fisher Scientific, cat. #11-0038-42, RRID: AB_2043831, 5 μL/1 × 10 6 cells), PE anti-human CD127 (BioLegend, cat. #351304, RRID: AB_10720185, 5 μL/1 × 10 6 cells), Brilliant Violet 421 anti-human CD294 (BioLegend, cat. #350112, RRID: AB_2562468, 5 μL/1 × 10 6 cells), APC anti-human CD117 (BioLegend, cat. #313206, RRID: AB_314985, 5 μL/1 × 10 6 cells), FITC anti-human CD19 (eBioscience, cat. #11-0199-42, RRID: AB_10669461, 5 μL/1 × 10 6 cells), FITC anti-human CD14 (eBioscience, cat. #11-0149-42, RRID: AB_10597597, 5 μL/1 × 10 6 cells), BV650 anti-human CD45 (eBioscience, cat. #416-0459-42, RRID: AB_2925684, 5 μL/1 × 10 6 cells), iFluor 647 anti-Ki67 (HUABIO, cat. #HA720163F, RRID: AB_3072100, 1 μL/1 × 10 6 cells), PE/Cyanine7 anti-human CD274 (Elabscience, cat. #E-AB-F1133H, 5 μL/1 × 10 6 cells), and PE anti-human CD273 (Elabscience, cat. #E-AB-F1175D, 5 μL/1 × 10 6 cells).

    Techniques: Knockdown, Injection, Imaging, Control, Flow Cytometry

    High expression of ABHD16A mediates the cumulative release of LysoPS into the TME. A, mIF images of the orthotopic gastric cancer (GC) tissues stained for PD-L1, RORC, and CD3. Yellow, PD-L1 + tumor cells; red, RORC + ILC3s; green, CD3 + cells. Scale bars, 100 μm (left) and 25 μm (right). B and C, Flow cytometry gating strategy and frequencies of ILC3s ( B ) and proportions of ILC3s in total CD45 + cells ( C ) isolated from control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tissues ( n = 3 per group). D and E, Flow cytometry gating strategy and frequencies of ILC3s ( D ) and proportions of ILC3s in total CD45 + cells ( E ) derived from the peripheral blood of healthy controls (HC) and patients with gastric cancer ( n = 8 per group). F and G, LC-MS/MS analysis of 18:0 and 18:1 LysoPS in the control and Abhd16a -knockdown orthotopic ( F ) and subcutaneous ( G ) gastric cancer tissues ( n = 3 per group). H–J, ELISA was used to measure levels of LysoPS in gastric cancer cell supernatant ( H ), TIF ( I ), and in vitro tumor culture supernatant ( J ) of control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tumors ( n = 3 per group). K, LysoPS levels in the serum of healthy controls and patients with gastric cancer detected by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Journal: Cancer Research

    Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

    doi: 10.1158/0008-5472.CAN-25-3092

    Figure Lengend Snippet: High expression of ABHD16A mediates the cumulative release of LysoPS into the TME. A, mIF images of the orthotopic gastric cancer (GC) tissues stained for PD-L1, RORC, and CD3. Yellow, PD-L1 + tumor cells; red, RORC + ILC3s; green, CD3 + cells. Scale bars, 100 μm (left) and 25 μm (right). B and C, Flow cytometry gating strategy and frequencies of ILC3s ( B ) and proportions of ILC3s in total CD45 + cells ( C ) isolated from control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tissues ( n = 3 per group). D and E, Flow cytometry gating strategy and frequencies of ILC3s ( D ) and proportions of ILC3s in total CD45 + cells ( E ) derived from the peripheral blood of healthy controls (HC) and patients with gastric cancer ( n = 8 per group). F and G, LC-MS/MS analysis of 18:0 and 18:1 LysoPS in the control and Abhd16a -knockdown orthotopic ( F ) and subcutaneous ( G ) gastric cancer tissues ( n = 3 per group). H–J, ELISA was used to measure levels of LysoPS in gastric cancer cell supernatant ( H ), TIF ( I ), and in vitro tumor culture supernatant ( J ) of control and Abhd16a -knockdown orthotopic and subcutaneous gastric cancer tumors ( n = 3 per group). K, LysoPS levels in the serum of healthy controls and patients with gastric cancer detected by ELISA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Article Snippet: FITC anti-human CD3 (Thermo Fisher Scientific, cat. #11-0038-42, RRID: AB_2043831, 5 μL/1 × 10 6 cells), PE anti-human CD127 (BioLegend, cat. #351304, RRID: AB_10720185, 5 μL/1 × 10 6 cells), Brilliant Violet 421 anti-human CD294 (BioLegend, cat. #350112, RRID: AB_2562468, 5 μL/1 × 10 6 cells), APC anti-human CD117 (BioLegend, cat. #313206, RRID: AB_314985, 5 μL/1 × 10 6 cells), FITC anti-human CD19 (eBioscience, cat. #11-0199-42, RRID: AB_10669461, 5 μL/1 × 10 6 cells), FITC anti-human CD14 (eBioscience, cat. #11-0149-42, RRID: AB_10597597, 5 μL/1 × 10 6 cells), BV650 anti-human CD45 (eBioscience, cat. #416-0459-42, RRID: AB_2925684, 5 μL/1 × 10 6 cells), iFluor 647 anti-Ki67 (HUABIO, cat. #HA720163F, RRID: AB_3072100, 1 μL/1 × 10 6 cells), PE/Cyanine7 anti-human CD274 (Elabscience, cat. #E-AB-F1133H, 5 μL/1 × 10 6 cells), and PE anti-human CD273 (Elabscience, cat. #E-AB-F1175D, 5 μL/1 × 10 6 cells).

    Techniques: Expressing, Staining, Flow Cytometry, Isolation, Control, Knockdown, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, In Vitro

    IL22 upregulates PD-L1 expression in gastric cancer cells through the UPR IRE1α–XBP1 axis. A, mIF images show the alterations of PD-L1 + tumor cells (purple), CD4 + (green), and CD8 + (red) T cells in orthotopic gastric cancer tumors from control and Abhd16a -knockdown mice following IL22 treatment. Scale bar, 50 μm. B, KEGG pathway enrichment analysis of RNA-seq data of gastric cancer tissues with or without IL22 treatment. C, RT-PCR was used to assess the mRNA expression of key downstream molecules of the UPR branches ( XBP1 , ATF4 , ATF6 ) in control and IL22RA1 -knockdown gastric cancer cells. D, Western blotting analysis of PD-L1 and XBP1s levels in control and IL22RA1 -knockdown MGC-803 cells treated with IL22 (100 μg/L). E, Western blotting detection of PD-L1 and XBP1s levels in XBP1- knockdown MGC-803 cells treated with IL22 and MGC-803 cells treated with IL22 or XBP1s inhibitor (STF083010, 30 μmol/L) in combination with IL22. F, The binding sequence of XBP1 on the CD274 promoter. G and H, ChIP ( G ) and luciferase reporter assay ( H ) showing the transcriptional regulation of CD274 by XBP1s under IL22 stimulation. I, Orthotopic gastric cancer mouse models ( n = 5 per group) were injected with anti-IL22 (200 μg per mouse), anti-CD90.2 antibody (150 μg per mouse), anti-CD90.2 antibody in combination with IL22 (500 ng per mouse), or anti-CD90.2 antibody in combination with XBP1s inhibitors (STF083010, 30 mg/kg) and IL22 for 2 weeks. IHC analysis was used to show IL22, XBP1s, and PD-L1 levels in gastric cancer tissues. Scale bar, 200 μm. J, Tumor volume of orthotopic gastric cancer models under treatments the same as in I . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

    Journal: Cancer Research

    Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

    doi: 10.1158/0008-5472.CAN-25-3092

    Figure Lengend Snippet: IL22 upregulates PD-L1 expression in gastric cancer cells through the UPR IRE1α–XBP1 axis. A, mIF images show the alterations of PD-L1 + tumor cells (purple), CD4 + (green), and CD8 + (red) T cells in orthotopic gastric cancer tumors from control and Abhd16a -knockdown mice following IL22 treatment. Scale bar, 50 μm. B, KEGG pathway enrichment analysis of RNA-seq data of gastric cancer tissues with or without IL22 treatment. C, RT-PCR was used to assess the mRNA expression of key downstream molecules of the UPR branches ( XBP1 , ATF4 , ATF6 ) in control and IL22RA1 -knockdown gastric cancer cells. D, Western blotting analysis of PD-L1 and XBP1s levels in control and IL22RA1 -knockdown MGC-803 cells treated with IL22 (100 μg/L). E, Western blotting detection of PD-L1 and XBP1s levels in XBP1- knockdown MGC-803 cells treated with IL22 and MGC-803 cells treated with IL22 or XBP1s inhibitor (STF083010, 30 μmol/L) in combination with IL22. F, The binding sequence of XBP1 on the CD274 promoter. G and H, ChIP ( G ) and luciferase reporter assay ( H ) showing the transcriptional regulation of CD274 by XBP1s under IL22 stimulation. I, Orthotopic gastric cancer mouse models ( n = 5 per group) were injected with anti-IL22 (200 μg per mouse), anti-CD90.2 antibody (150 μg per mouse), anti-CD90.2 antibody in combination with IL22 (500 ng per mouse), or anti-CD90.2 antibody in combination with XBP1s inhibitors (STF083010, 30 mg/kg) and IL22 for 2 weeks. IHC analysis was used to show IL22, XBP1s, and PD-L1 levels in gastric cancer tissues. Scale bar, 200 μm. J, Tumor volume of orthotopic gastric cancer models under treatments the same as in I . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

    Article Snippet: FITC anti-human CD3 (Thermo Fisher Scientific, cat. #11-0038-42, RRID: AB_2043831, 5 μL/1 × 10 6 cells), PE anti-human CD127 (BioLegend, cat. #351304, RRID: AB_10720185, 5 μL/1 × 10 6 cells), Brilliant Violet 421 anti-human CD294 (BioLegend, cat. #350112, RRID: AB_2562468, 5 μL/1 × 10 6 cells), APC anti-human CD117 (BioLegend, cat. #313206, RRID: AB_314985, 5 μL/1 × 10 6 cells), FITC anti-human CD19 (eBioscience, cat. #11-0199-42, RRID: AB_10669461, 5 μL/1 × 10 6 cells), FITC anti-human CD14 (eBioscience, cat. #11-0149-42, RRID: AB_10597597, 5 μL/1 × 10 6 cells), BV650 anti-human CD45 (eBioscience, cat. #416-0459-42, RRID: AB_2925684, 5 μL/1 × 10 6 cells), iFluor 647 anti-Ki67 (HUABIO, cat. #HA720163F, RRID: AB_3072100, 1 μL/1 × 10 6 cells), PE/Cyanine7 anti-human CD274 (Elabscience, cat. #E-AB-F1133H, 5 μL/1 × 10 6 cells), and PE anti-human CD273 (Elabscience, cat. #E-AB-F1175D, 5 μL/1 × 10 6 cells).

    Techniques: Expressing, Control, Knockdown, RNA Sequencing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Binding Assay, Sequencing, Luciferase, Reporter Assay, Injection

    Combination therapy enhances the anti–PD-L1 immunotherapeutic effect in gastric cancer. A and B, After tumor formation, the orthotopic gastric cancer mice ( n = 5 per group) were treated with anti–PD-L1 (100 μg per mouse), GPR34 inhibitor (20 mg/kg), or XBP1s inhibitor (30 mg/kg) every 3 days or ACh inhibitor (2.5 mg/kg) daily. Combinations of anti–PD-L1 with each inhibitor followed the every 3-day dosing schedule for a total duration of 2 weeks via i.p. injection. Living images were used to monitor tumor progression at 5-day intervals from the time of drug administration ( A ); IHC and mIF were performed to detect PD-L1 and XBP1s levels and proportions of CD4 + (green) and CD8 + (red) T cells in gastric cancer tissues at the end of treatments ( B ). Scale bars, 1.000e+5 –∼ 5.000e + 5 p/s/cm 2 /sr for living images; 200 μm for IHC; 50 μm for immunofluorescence. C and D, Representative images ( C ) and tumor volume ( D ) of subcutaneous tumors. The administration protocol for the mice was consistent with the description provided in A and B . **, P < 0.01; ***, P < 0.001.

    Journal: Cancer Research

    Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

    doi: 10.1158/0008-5472.CAN-25-3092

    Figure Lengend Snippet: Combination therapy enhances the anti–PD-L1 immunotherapeutic effect in gastric cancer. A and B, After tumor formation, the orthotopic gastric cancer mice ( n = 5 per group) were treated with anti–PD-L1 (100 μg per mouse), GPR34 inhibitor (20 mg/kg), or XBP1s inhibitor (30 mg/kg) every 3 days or ACh inhibitor (2.5 mg/kg) daily. Combinations of anti–PD-L1 with each inhibitor followed the every 3-day dosing schedule for a total duration of 2 weeks via i.p. injection. Living images were used to monitor tumor progression at 5-day intervals from the time of drug administration ( A ); IHC and mIF were performed to detect PD-L1 and XBP1s levels and proportions of CD4 + (green) and CD8 + (red) T cells in gastric cancer tissues at the end of treatments ( B ). Scale bars, 1.000e+5 –∼ 5.000e + 5 p/s/cm 2 /sr for living images; 200 μm for IHC; 50 μm for immunofluorescence. C and D, Representative images ( C ) and tumor volume ( D ) of subcutaneous tumors. The administration protocol for the mice was consistent with the description provided in A and B . **, P < 0.01; ***, P < 0.001.

    Article Snippet: FITC anti-human CD3 (Thermo Fisher Scientific, cat. #11-0038-42, RRID: AB_2043831, 5 μL/1 × 10 6 cells), PE anti-human CD127 (BioLegend, cat. #351304, RRID: AB_10720185, 5 μL/1 × 10 6 cells), Brilliant Violet 421 anti-human CD294 (BioLegend, cat. #350112, RRID: AB_2562468, 5 μL/1 × 10 6 cells), APC anti-human CD117 (BioLegend, cat. #313206, RRID: AB_314985, 5 μL/1 × 10 6 cells), FITC anti-human CD19 (eBioscience, cat. #11-0199-42, RRID: AB_10669461, 5 μL/1 × 10 6 cells), FITC anti-human CD14 (eBioscience, cat. #11-0149-42, RRID: AB_10597597, 5 μL/1 × 10 6 cells), BV650 anti-human CD45 (eBioscience, cat. #416-0459-42, RRID: AB_2925684, 5 μL/1 × 10 6 cells), iFluor 647 anti-Ki67 (HUABIO, cat. #HA720163F, RRID: AB_3072100, 1 μL/1 × 10 6 cells), PE/Cyanine7 anti-human CD274 (Elabscience, cat. #E-AB-F1133H, 5 μL/1 × 10 6 cells), and PE anti-human CD273 (Elabscience, cat. #E-AB-F1175D, 5 μL/1 × 10 6 cells).

    Techniques: Injection, Immunofluorescence